13C nuclear magnetic resonance analysis of glucose and citrate end products in an ldhL-ldhD double-knockout strain of Lactobacillus plantarum.
نویسندگان
چکیده
We have examined the metabolic consequences of knocking out the two ldh genes in Lactobacillus plantarum using 13C nuclear magnetic resonance. Unlike its wild-type isogenic progenitor, which produced lactate as the major metabolite under all conditions tested, ldh null strain TF103 mainly produced acetoin. A variety of secondary end products were also found, including organic acids (acetate, succinate, pyruvate, and lactate), ethanol, 2,3-butanediol, and mannitol.
منابع مشابه
Nuclear Magnetic Resonance Analysis of Glucose and Citrate End Products in an ldhL-ldhD Double-Knockout Strain of Lactobacillus plantarum
We have examined the metabolic consequences of knocking out the two ldh genes in Lactobacillus plantarum using C nuclear magnetic resonance. Unlike its wild-type isogenic progenitor, which produced lactate as the major metabolite under all conditions tested, ldh null strain TF103 mainly produced acetoin. A variety of secondary end products were also found, including organic acids (acetate, succ...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 178 24 شماره
صفحات -
تاریخ انتشار 1996